Effects of a variety of treatments affecting Chinese cabbage protoplast culture, and plant regeneration from protoplast-derived callus
نویسندگان
چکیده
منابع مشابه
Regeneration of Stevia Plant Through Callus Culture
Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...
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Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described.
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Method for production and regeneration of Acetobacter pasterianus protoplasts is described. The protoplasts were obtained by treatment of cells with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with NaCl (0.5M) as ...
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The existence of beta-lectins on protoplast surfaces is confirmed by the agglutination of protoplasts by those Yariv antigens that have sugar specificities which also interact with isolated beta-lectins. Agglutination by beta-maltosyl but not by beta-D-mannosyl Yariv antigens is used to identify some of the structural features required of the ligand for beta-lectin binding. Inhibition of agglut...
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To regenerate auxotrophic mutants of Physcomitrella patens, two media of increasing complexity were developed. The survival rate of protoplasts was around 30% higher on full medium when compared to standard minimal medium. Protoplast survival was higher in a medium containing 2.5 mmol/L ammonium tartrate compared to a medium with 5 mmol/L of this compound. Solid medium had a positive effect on ...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2008
ISSN: 1229-2818
DOI: 10.5010/jpb.2008.35.3.235